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張翠軍課題組

張翠軍課題組

Zhang Cuijun Lab

  課題組長

張翠軍,,研究員,,博導,。2010年碩博連讀畢業(yè)于河北師范大學,,其后相繼在北京生命科學研究所,、上海植物逆境生物學研究中心、美國普渡大學開展研究,2019年加入農(nóng)業(yè)基因組所。其課題組利用基因組學,、遺傳學、生化與分子生物學等多學科手段開展小麥抗赤霉病及基因編輯改良研究。重點研究: 1) 禾谷鐮刀菌與小麥互作,;2) 小麥抗赤霉病機理及新基因挖掘,;3)小麥基因編輯改良。在 Nature Communications, The EMBO Journal, Cell Research, Plant Biotechnology Journal, J Integr Plant Biol等國際一流期刊上發(fā)表多篇論文,。

聯(lián)系方式:[email protected],。


  工作經(jīng)歷

  中國農(nóng)業(yè)科學院農(nóng)業(yè)基因組研究所        研究員            2019.10–至今

  中國科學院植物研究所                        研究員             2019.5–2019.9

  普渡大學                                            博士后             2015.8–2019.4

  上海植物逆境生物學研究中心              副研究員          2015.4–2015.8

  北京生命科學研究所                           研究助理          2010.7–2015.4

 

  教育經(jīng)歷

  河北師范大學                            博士&碩士           2005.9–2010.6

  河北師范大學                            本科                    2001.9–2005.6

 

  團隊研究方向

1.小麥赤霉病是小麥最嚴重的病害之一,主要由禾谷鐮刀菌 (Fusarium graminearum) 引起,,每年給小麥生產(chǎn)造成巨大損失,,并導致小麥籽粒毒素污染,對人類和牲畜健康構(gòu)成嚴重威脅,。近幾年氣候變化和耕作制度改變,,赤霉病的發(fā)生具有愈來愈嚴重的趨勢。我們聯(lián)合應用多種技術(shù)對小麥赤霉病發(fā)病機制及小麥抗赤霉病機理開展深入研究,,并對特殊小麥品系赤霉病抗性進行評價,篩選小麥赤霉病新抗源,,克隆新的關(guān)鍵抗病基因,,為小麥抗赤霉病改良提供基礎(chǔ);

2.普通小麥為異源六倍體,,復雜的基因組限制了小麥功能基因的發(fā)現(xiàn),。基因編輯技術(shù)在多倍體作物功能基因發(fā)現(xiàn)及遺傳改良方面具有巨大優(yōu)勢,,我們應用基因編輯技術(shù)對小麥環(huán)境適應性及抗性進行高效改良,。

 

  研究進展

1.通過質(zhì)譜對禾谷鐮刀菌的分泌蛋白組進行了研究,發(fā)現(xiàn)了多個能夠引起植物細胞死亡的分泌性蛋白,,其中發(fā)現(xiàn)五個枯草桿菌蛋白酶(Subtilisin-like family proteases, FgSLPs)作為毒素蛋白能夠引物植物非特異性細胞死亡,,是禾谷鐮刀菌致病關(guān)鍵蛋白。

2.通過研究小麥與禾谷鐮刀菌互作,,發(fā)現(xiàn)了多個禾谷鐮刀菌致病性效應子蛋白及其小麥中的靶蛋白,,揭示了禾谷鐮刀菌致病性效應子蛋白介導的新型致病機制。

3.通過 CRISPR/Cas9 基因編輯技術(shù)修復Tamyb10-B1a基因的移碼突變,,使Tamyb10-B1基因恢復功能,,提高了小麥穗發(fā)芽抗性。


發(fā)表論文

1. Shengping Shang; Yuhan He; Qianyong Hu; Ying Fang; Shifeng Cheng; Cui-Jun Zhang* (2024). Fusarium graminearum effector FgEC1 targets wheat TaGF14b protein to suppress TaRBOHD‐mediated ROS production and promote infection. J Integr Plant Biol., doi.org/10.1111/jipb.13752

2. Xishi Zhou, Yujin Fan, Xiying Zhu, Ruihua Zhao, Junna He, Pengfeng Li, Shengping Shang, Justin Goodrich, Jian-Kang Zhu, Cui-Jun Zhang* (2024). SANT proteins modulate gene expression by coordinating histone H3KAc and Khib levels and regulate plant heat tolerance. Plant Physiology, doi.org/10.1093/plphys/kiae348.

3. Jiang Xiong, Mingyu Luo, Yunshen Chen, Qianyong Hu, Ying Fang, Tongjun Sun, Guanjing Hu, Cui-Jun Zhang* (2024). Subtilisin-like proteases from Fusarium graminearum induce plant cell death and contribute to virulence. Plant Physiology, 195, 1681–1693.

4. Yiwang Zhu#, Yarong Lin#, Yujin Fan, Yiwei Wang, Pengfeng Li, Jiang Xiong, Yuhan He, Shifeng Cheng, Xingguo Ye, Feng Wang, Justin Goodrich, Jian-Kang Zhu, Ke Wang*, and Cui-Jun Zhang* (2023). CRISPR/Cas9-mediated restoration of Tamyb10 to create pre-harvest sprouting-resistant red wheat. Plant Biotechnology Journal, 21, 665–667.

5. Lina Zhang#, Xishi Zhou#, Pengfeng Li, Yiwei Wang, Qianyong Hu, Yuping Shang, Yunshen Chen, Xiying Zhu, Hongjie Feng*, and Cui-Jun Zhang* (2023). Transcriptome Profile of Fusarium graminearum Treated by Putrescine. J. Fungi, 9, 60.

6. Yarong Lin#, Yiwang Zhu#, Yuchao Cui#, Hongge Qian#, Qiaoling Yuan#, Rui Chen, Yan Lin, Jianmin Chen, Xishi Zhou, Chuanlin Shi, Huiying He, Taijiao Hu, Chenbo Gu, Xiaoman Yu, Xiying Zhu, Yuexing Wang, Qian Qian, Cui-Jun Zhang*, Feng Wang*, and Lianguang Shang* (2023). Identification of natural allelic variation in TTL1 controlling thermotolerance and grain size by a rice super pan‐genome. J Integr Plant Biol., 65: 2541–2551.

7. Xishi Zhou#, Junna He#, Christos N. Velanis#, Yiwang Zhu, Yuhan He, Kai Tang, Mingku Zhu, Lisa Graser, Erica de Leau, Xingang Wang, Lingrui Zhang, W. Andy Tao, Justin Goodrich*, Jian-Kang Zhu*, Cui-Jun Zhang* (2021). A domesticated Harbinger transposase forms a complex with HDA6 and promotes histone H3 deacetylation at genes but not TEs in Arabidopsis. J Integr Plant Biol., 63, 1462–1474.

8. Cuijun Zhang#, Xuan Du#, Kai Tang#, Zhenlin Yang, Li Pan, Peipei Zhu, Jinyan Luo, Yuwei Jiang,Hui Zhang, Huafang Wan, Xingang Wang, Fengkai Wu, W. Andy Tao, Xin-Jian He, Heng Zhang, Ray A. Bressan, Jiamu Du*, Jian-Kang Zhu* (2018). Arabidopsis AGDP1 links H3K9me2 to DNA methylation in heterochromatin. Nature Communications, 9, 4547.

9. Lian-Mei Tan#, Cui-Jun Zhang#, Xiao-Mei Hou, Chang-Rong Shao, Yu-Jia Lu, Jin-Xing Zhou, Yong-Qiang Li, Lin Li, She Chen, Xin-Jian He* (2018). The PEAT protein complexes are required for histone deacetylation and heterochromatin silencing. EMBO Journal, 37, e100573.

10. Cui-Jun Zhang#, Xiao-Mei Hou#, Lian-Mei Tan, Chang-Rong Shao, Huan-Wei Huang, Yong-Qiang Li, Lin Li, Tao Cai, She Chen, Xin-Jian He* (2016). The Arabidopsis acetylated histone-binding protein BRAT1 forms a complex with BRP1 and prevents transcriptional silencing. Nature Communications, 7, 11715.

11. Cui-Jun Zhang#, Jin-Xing Zhou#, Jun Liu#, Ze-Yang Ma, Su-Wei Zhang, Kun Dou, Huan-Wei Huang, Tao Cai, Renyi Liu, Jian-Kang Zhu, Xin-Jian He* (2013). The splicing machinery promotes RNA-directed DNA methylation and transcriptional silencing in Arabidopsis. EMBO Journal, 32, 1128-40.

12. Cui-Jun Zhang, Yong-Qiang Ning, Su-Wei Zhang, Qing Chen, Chang-Rong Shao, Yan-Wu Guo, Jin-Xing Zhou, Lin Li, She Chen, Xin-Jian He* (2012). IDN2 and its paralogs form a complex required for RNA-directed DNA methylation. PLoS Genetics, 8, e1002693.

13. Jun Liu#, Ge Bai#, Cuijun Zhang#, Wei Chen, Jinxing Zhou, Suwei Zhang, Qing Chen, Xin Deng, Xin-Jian He*, Jian-Kang Zhu (2011). An atypical component of RNA-directed DNA methylation machinery has both DNA methylation-dependent and-independent roles in locus-specific transcriptional gene silencing. Cell Research, 21, 1691-1700.

14. Cuijun Zhang, and Yi Guo* (2012). OsTRXh1 regulates the redox state of the apoplast and influences stress responses in rice. Plant Signal Behav., 7, 441-443.

15. Cui-Jun Zhang#, Bing-Chun Zhao#, Wei-Na Ge, Ya-Fang Zhang, Yun Song, Da-Ye Sun, and Yi Guo* (2011). An apoplastic h-type thioredoxin is involved in the stress response through regulation of the apoplastic reactive oxygen species in rice. Plant Physiology, 157, 1884-1899.

16. Yun Song#, Cuijun Zhang#, Weina Ge, Yafang Zhang, Alma L. Burlingame, Yi Guo* (2011). Identification of NaCl stress-responsive apoplastic proteins in rice shoot stems by 2D-DIGE. J Proteomics, 74, 1045-1067.


  張翠軍課題組更新于2024年8月

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