簡歷介紹

課題組組長

萬方浩,，研究員,，博士生導(dǎo)師。

1989年畢業(yè)于中國農(nóng)業(yè)科學(xué)院研究生院,，獲理學(xué)博士學(xué)位,。1984-2018年先后在湖南農(nóng)學(xué)院和中國農(nóng)業(yè)科學(xué)院生物防治研究所、植物保護(hù)研究所從事入侵生物學(xué)研究,。目前主要從事生物入侵及生物防治的研究,，創(chuàng)建了我國入侵生物學(xué)新興學(xué)科。先后主持國家“973”計劃等國家重點項目20余項,；獲國家和省部級成果獎20項（國家科技進(jìn)步二等獎2項）,；發(fā)表論文700余篇(SCI-200余篇)，出版專著21部,。

聯(lián)系方式：[email protected]

錢萬強(qiáng),，研究員,、博士生導(dǎo)師,，兼任中國植保學(xué)會入侵生物學(xué)分會副主任,。

2006年畢業(yè)于北京大學(xué)，獲理學(xué)博士學(xué)位,。2014至今一直在中國農(nóng)業(yè)科學(xué)院農(nóng)業(yè)基因組研究所從事入侵生物學(xué)研究,。主要研究方向為入侵生物學(xué)、植物保護(hù),，運用生物多組學(xué)技術(shù),、人工智能技術(shù)，揭示薇甘菊等入侵物種傳入與暴發(fā)的分子機(jī)制,，豐富了“內(nèi)稟優(yōu)勢”“適應(yīng)性演化”等入侵假說,；研發(fā)了入侵種高光譜識別技術(shù)、入侵生物基因組數(shù)據(jù)庫等檢測監(jiān)測技術(shù)方法和平臺,，為行業(yè)部門制定國家重點管理入侵物種名錄和區(qū)域性擴(kuò)散前沿的調(diào)查監(jiān)測提供了支撐,。近 5 年來，以第一或通訊作者在 Nature Communications等雜志上發(fā)表 SCI論文 19 余篇,，其中中科院 1 區(qū)論文10篇,；授權(quán)國家發(fā)明專利 4 項，主持國家重點研發(fā)計劃課題等國家級科研項目 6 項,，主持發(fā)起IAS1000（國際千種入侵生物基因組計劃）,。

聯(lián)系方式：[email protected]

團(tuán)隊成員

姓名	職稱	聯(lián)系方式
劉博	副研究員，碩士生導(dǎo)師,，深圳市鵬城孔雀計劃C檔特聘	[email protected]
喬曦	副研究員,，碩士生導(dǎo)師，深圳市鵬城孔雀計劃C檔特聘	[email protected]
劉聰輝	副研究員,，碩士生導(dǎo)師	[email protected]
宋新月	科研助理	[email protected]

 研究領(lǐng)域：

研究內(nèi)容

圍繞重要農(nóng)業(yè)入侵生物防控基因組及基于組學(xué)大數(shù)據(jù)深度分析,，開展深層次的入侵機(jī)制與防控新技術(shù)的研發(fā)。利用現(xiàn)代基因組測序技術(shù)和組裝策略構(gòu)建高質(zhì)量的入侵生物基因組,，結(jié)合生物學(xué)實驗解析入侵的分子機(jī)制與生態(tài)學(xué)過程,，挖掘重要入侵性相關(guān)基因，進(jìn)而開發(fā)相應(yīng)基因干擾的分子農(nóng)藥和智能檢測監(jiān)測系統(tǒng),，創(chuàng)新入侵生物防控新技術(shù),。

研究進(jìn)展

1.薇甘菊入侵機(jī)制與生物防治

薇甘菊可在白天和夜晚分別利用C3和C4的光合途徑進(jìn)行CO₂的固定，提高了碳固定效率,；從薇甘菊葉5種賈澤蘭屬特異的倍半萜內(nèi)酯單體化合物對薇甘菊土壤氮循環(huán)菌的富集有顯著的促進(jìn)作用,，為其快速生長提供了充足的營養(yǎng)物質(zhì)。此外,，發(fā)現(xiàn)薇甘菊專性病原真菌（Puccinia spegazzinii）侵染薇甘菊后,，不僅可降低生長激素水平,，還可減弱光合作用能力。該研究深入揭示了專一性柄銹菌通過控制薇甘菊體內(nèi)茉莉酸水平來抑制其生長的分子機(jī)制,。

1. Mikania micrantha invasion mechanism and biological control

Mikania micrantha could use C3 and C4 photosynthetic pathways to fix CO2 during the day and night respectively, which improved the efficiency of carbon fixation. Five kinds of sesquiterpene lactones from the leaves of M. micrantha significantly promoted the enrichment of relative abundance in soil nitrogen cycling bacteria, which provided sufficient nutrients for M. micrantha rapid growth. In addition, after host-specific rust (Puccinia spegazzinii) infected Mikania micrantha, not only reducing the level of growth hormone, but also inhibiting the photosynthetic capacity in M. micrantha leaves. The results reveal that inhibition mechanism of invasive plant Mikania micrantha rapid growth by host-specific rust.

2. 利用組學(xué)技術(shù)解析蘋果蠹蛾適應(yīng)性進(jìn)化機(jī)制,，探索開發(fā)新型防控制劑

通過基因組學(xué)和比較基因組學(xué)發(fā)現(xiàn)，蘋果蠹蛾共編碼40個氣味結(jié)合蛋白 (Odorant Binding Proteins, OBPs),，其擴(kuò)張主要由基因串聯(lián)拷貝導(dǎo)致,，進(jìn)一步發(fā)現(xiàn)CpomGOBP1和CpomOBP20與蘋果蠹蛾寄主識別及生長發(fā)育相關(guān)。通過病毒侵染轉(zhuǎn)錄組分析蘋果蠹蛾顆粒體病毒（Cydia pomonella granulovirus, CpGV）侵染宿主不同時間點病毒基因復(fù)制的時空表達(dá)模式,，揭示了病毒侵染復(fù)制過程,。

2. Utilize omics technologies to unravel the adaptive evolutionary mechanism of codling moth and explore the development of novel pest control agents

Through genomics and comparative genomics analysis, we identified 40 odorant binding proteins (OBPs) in codling moth, and found that the expansion of OBPs is mainly caused by tandem duplications. Further research revealed that CpomGOBP1 and CpomOBP20 are associated with host recognition and growth and development of codling moth. The spatial and temporal expression patterns of viral genes were analyzed through the transcriptome of codling moth at different time points after infected by Cydia pomonella granulovirus (CpGV), which revealed the process of viral infection and replication.

3.入侵軟體動物入侵機(jī)制及檢測、防控策略

研究團(tuán)隊構(gòu)建了染色體級的福壽螺和非洲大蝸?；蚪M,，開展了比較基因組學(xué)分析，獲得重要入侵性相關(guān)基因并進(jìn)行了功能驗證,，并對不同地理種群開展了基因組重測序分析,，從基因組水平解析了其入侵的分子機(jī)制。開發(fā)了靈敏型eDNA檢測技術(shù),，為水生入侵生物的入侵提供監(jiān)測和預(yù)警,。利用入侵植物的化感物質(zhì)和蝸牛信息素開發(fā)了植物源誘殺劑。開發(fā)了入侵軟體動物的RNAi農(nóng)藥和功能驗證平臺,。

3. Invasion mechanism, detection and control strategies of invasive mollusks

The genomes of golden apple snail and giant African snails at the chromosome level were constructed, comparative genomics analysis was carried out, important invasive related genes were obtained and Functional verification was carried out, genome re sequencing analysis was carried out for different geographical populations, and the molecular mechanism of their invasion was analyzed at the genome level. Sensitive eDNA detection technology was developed to provide monitoring and early warning for the invasion of aquatic invasive species. The allelochemicals of invasive plants and snail pheromone were employed to develop plant derived molluscicides. RNAi molluscicides and functional verification platform were developed for molluscs invasion.

4.外來入侵生物智能監(jiān)測

利用多光譜和高清相機(jī)采集外來入侵生物表型數(shù)據(jù),，構(gòu)建多尺度的外來入侵生物智能識別模型，基于識別結(jié)果開發(fā)入侵植物關(guān)鍵植被參數(shù)預(yù)測模型,，結(jié)合入侵植物生物學(xué)特性和環(huán)境因子,，成功構(gòu)建了100余種外來入侵植物的擴(kuò)散及危害預(yù)警系統(tǒng)。此外,，還開發(fā)了基于入侵昆蟲識別的田間害蟲檢測微信小程序和入侵雜草種子檢測裝備,。

4. Invasive alien species intelligent monitoring

We used multi-spectral and high-definition cameras for phenotype data collection of invasive alien species, and constructed a multi-scale intelligent identification model of invasive alien species. Then, a prediction model of key vegetation parameters of invasive plants was developed based on the identification results of invasive plant. An early warning system was successfully built for the spread and damage of more than 100 invasive alien plants by combining the biological characteristics of invasive plants and environmental factors. In addition, we have developed a WeChat applet for pest detection in field based on invasive insect identification, and a seed detection equipment for invasive plant seed.

5. 外來入侵物種基因組與基因數(shù)據(jù)的綜合分析數(shù)據(jù)庫平臺構(gòu)建

通過已完成基因組測序的外來入侵物種的組學(xué)相關(guān)數(shù)據(jù)，構(gòu)建了InvasionDB數(shù)據(jù)庫,，為從基因組水平探究外來物種的入侵性,，以及發(fā)展新的防控技術(shù)具有重要指導(dǎo)意義。通過基因組和轉(zhuǎn)錄組數(shù)據(jù),，構(gòu)建了蘋果蠹蛾lncRNA分析流程及新基因鑒定方法,，為進(jìn)一步研究蘋果蠹蛾各種生理活動分子機(jī)制提供了數(shù)據(jù)支撐。

5. Development of a comprehensive analytical database platform for genetic and genomic data of invasive alien species

We constructed the InvasionDB database by utilizing the omics-related data of invasive alien species with completed genome sequencing. This database provides significant guidance for investigating the invasiveness of alien species at the genomic level, as well as developing new prevention and control techniques. Based on the genomic and transcriptomic data, we developed an analysis workflow for lncRNA and a new gene identification method for the codling moth (Cydia pomonella). These findings provide essential data support for further research on the molecular mechanism underlying various physiological activities of the codling moth.

6. IAS1000計劃

IAS1000計劃（1000種入侵物種基因組計劃）順利推進(jìn),，目前與40余家科研院所,、大學(xué)與企業(yè)建立了合作關(guān)系。研究團(tuán)隊已完成首批確定的重要入侵物種名單，包括薇甘菊,、紫莖澤蘭,、蘋果蠹蛾、紅脂大小蠹,、甜菜夜蛾,、福壽螺、非洲大蝸牛,、潛葉蛾,、蘋果綿蚜,、稻水象甲和椰心葉甲等物種展開了測序和基因組分析,。

6. IAS1000 Project

The IAS1000 Program (the Genome project of 1000 invasive species) has been successfully carried out. At present, it has established cooperative relationships with more than 40 scientific research institutes, universities and enterprises. The research team has completed the first list of identified important invasive species, including Mikania micrantha, Eupatorium adenophorum, Cydia pomonella, Dendroctonus valens, Spodoptera exigua, Pomacea canaliculata, Achatina immaculate, Tuta sp., Eriosoma lanigerum, Lissorhoptrus oryzophilus, and Brontispa longissima. More invasive species has conducted sequencing and genome analysis.

承擔(dān)項目（錢萬強(qiáng)）

2021.12-2024.11重大入侵種的入侵過程遺傳適應(yīng)性進(jìn)化機(jī)制（國家重點研發(fā)計劃，2021YFC2600101,，主持人）

2022.07-2022.12 外來入侵物種鑒定和標(biāo)本制作等（農(nóng)業(yè)農(nóng)村部科技教育司,，13220177，主持人）

2021.11-2022.03 普查點位調(diào)查數(shù)據(jù)匯總分析及技術(shù)指導(dǎo)等（農(nóng)業(yè)農(nóng)村部科技教育司,，13210396,，主持人）

2021.01-2021.06 外來入侵物種普查試點技術(shù)支撐服務(wù)（農(nóng)業(yè)農(nóng)村部科技教育司，13200449,，主持人）

2020.01-2022.12 開發(fā)基于重大入侵生物宏基因組分析的下一代防控策略（中德科學(xué)基金研究交流中心,，M-0050，主持人）

2016.07-2018.12重要入侵生物的基因組特征及遺傳結(jié)構(gòu)（國家重點研發(fā)計劃,，2016YFC1200602 主持人）

 代表論著：

代表論文：

1. Liu B?, Yan J?, Li WH?, Yin LJ?, Li P?, Yu HX, …, Dong H, Peng CL*, Qian WQ*, Fan W*, Wan FH*. 2019. Mikania micrantha genome provides insights into the molecular mechanism of rapid growth. Nature Communications, 11(1):340.

2. Wan FH *?, Yin CL?, Tang R?, Chen MH?, Wu Q?, Huang C?, Qian WQ, …, Walters James*, Li F*. 2019. A chromosome-level genome assembly of Cydia pomonella provides insights into chemical ecology and insecticide resistance. Nature Communications, 10:4237.

3. Zhang X?, Liu B?, Zou F, Shen DY, Yin ZY, Wang RB, He F, Wang YC, Tyler BM, Fan W*, Qian WQ*, Dou DL*. 2019, Whole Genome Re-sequencing Reveals Natural Variation and Adaptive Evolution of Phytophthora sojae. Frontiers in Microbiology, 10:2792.

4. Huang C, Wu Q, Jiang CY, Xing LS, Shi GL, Zhang B, Qian WQ, Li YZ, Xi Y, Yang NW*, Wan FH*. 2019. Identification and developmental expression of putative gene encoding juvenile hormone esterase (CpJHE-like) in codling moth, Cydia pomonella (L.). Journal of Integrative Agriculture, 18(7): 2-11.

5. Liu CH?, Ren YW?, Li ZY?, Hu Q, Yin LJ, Wang HC, Qiao X, Zhang Y, Xing LS, Xi Y, Jiang F, Wang S, Huang C, Liu B, Liu HW, Wan FH, Qian WQ*, Fan W*. 2020. Giant African snail genomes provide insights into molluscan whole-genome duplication and aquatic-terrestrial transition. Molecular Ecology Resources, 10.1111/1755-0998.13261

6. Zhang L?, Liu B?, Zheng WG?; Liu CH, Zhang DD, Zhao SY, Li ZY, Xu PJ, …, Wang GR, Fan W*, Qian WQ*, Wu KM*, Xiao YT*. 2020. Genetic structure and insecticide resistance characteristics of fall armyworm populations invading China. Molecular Ecology Resources, 10.1111/1755-0998.13219

7. Yin LJ?, Liu B?, Wang HC, Zhang Y, Wang S, Jiang F, Ren YW, Liu HW, Liu CH, Wan FH, Wang HH, Qian WQ*, Fan W*. 2020. The Rhizosphere Microbiome of Mikania micrantha Provides Insight Into Adaptation and Invasion. Frontiers in Microbiology, 11:1462.

8. Qian WQ?, Huang YQ?, Liu Q?, Fan W, Sun ZY, Dong H, Wan FH*, Qiao X*. 2020. UAV and a deep convolutional neural network for monitoring invasive alien plants in the wild. Computers and Electronics in Agriculture, 174(2020):105519.

9. Qiao X?, Li YZ?, Su GY?, Tian HK, Zhang S, Sun ZY., Yang L, Wan FH., Qian WQ*. 2020. MmNet: Identifying Mikania micrantha Kunth in the wild via a deep convolutional neural network. Journal of Integrative Agriculture, 19(5): 1292-1300.

10. Xing LS.?, Xi Y.?, Qiao X., Huang C., Wu Q., Yang NW., Guo JY., Liu WX., Fan W.*, Wan FH.*, Qian WQ.*. 2021. The landscape of lncRNAs in Cydia pomonella provides insights into their signatures and potential roles in transcriptional regulation. BMC Genomics, 22(1): 4.

11. Xi Y. ?, Xing LS. ?, J?rg Wennmann T., Fan JB., Li ZY., Wu Q., Lu S., Liu B., Guo JY., Qiao X., Huang C., Qian WQ.*, Johannes A.Jehle*, Wan FH.*. 2021. Gene expression patterns of Cydia pomonella granulovirus in codling moth larvae revealed by RNAseq analysis. Virology, 558: 110-118.

12. Huang YQ.?, Li J.?, Yang R.?, Wang FK., Li YZ., Zhang S., Wan FH.* , Qiao X.*, Qian WQ.*. 2021. Hyperspectral imaging for identification of an invasive plant Mikania micrantha Kunth. Frontiers in plant science, 12: 626516.

13. Cong Huang?, Xue Zhang?, Dongfeng He, Qiang Wu, Rui Tang, Longsheng Xing, Wanxue Liu, Wenkai Wang, Bo Liu, Yu Xi, Nianwan Yang*, Fanghao Wan*, Wanqiang Qian*. 2021. Comparative Genomics Provide Insights Into Function and Evolution of Odorant Binding Proteins in Cydia pomonella. Frontiers in Physiology, 12: 690185.

14. Huang C.?, Lang K.?, Qian WQ.?, Wang SP., Cao XM., He R., Zhang AR., Chen MY., Yang NW.*, Li F.. 2021. InvasionDB: A genome and gene database of invasive alien species. Journal of Integrative Agriculture, 20(1): 191-200.

15. Wan J.?, Huang C.?, Li CY., Zhou HX., Ren YL., Li ZY., Xing LS., Zhang B., Qiao X., Liu B., Liu CH., Xi Y., Liu WX., Wang WK., Qian, WQ., Mckirdy S*, Wan FH.*. 2021. Biology, invasion and management of the agricultural invader: Fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae). Journal of Integrative Agriculture, 2021, 20(3): 646-663.

16. Zhudong Liu?, Longsheng Xing?, Wanlong Huang, Bo Liu, Fanghao Wan, Kenneth F. Raffa, Richard W. Hofstetter, Wanqiang Qian*, Jianghua Sun*. 2022. Chromosome?level genome assembly and population genomic analyses provide insights into adaptive evolution of the red turpentine beetle, Dendroctonus valens. BMC Biology, 20: 190.

17. Jing Wang?, Jianguang Li?, Zaiyuan Li, Bo Liu, Lili Zhang, Dongliang Guo, Shilian Huang, Wanqiang Qian*, Li Guo*.2022. Genomic insights into longan evolution from a chromosome-level genome assembly and population genomics of longan accessions. Horticulture Research, 9: uhac021.

18. Ping Li?, Genxiang Bai?, Jiangbin He?, Bo Liu?, Junru Long?, Taylan Morcol, Weiyao Peng, Fan Quan, Xinbo Luan, Zhenzhen Wang, Yi Zhao, Yunsheng Cha, Yuanyuan Liu, Juncai He, Lianzhang Wu, Yi Yang, Edward J. Kennelly, Quan Yang*, Lirong Sun*, Zepeng Chen, Wanqiang Qian*, Jian Hu*, ian Yan*. 2022. Chromosome-level genome assembly of Amomum tsao-ko provides insights into the biosynthesis of flavor compounds. Horticulture Research, 9: uhac211.

19. Longsheng Xing, Qiang Wu, Yu Xi, Cong Huang, Wanxue Liu, Fanghao Wan*, Wanqiang Qian*. 2022. Full-length codling moth transcriptome atlas revealed by single-molecule real-time sequencing. Genomics, 114, (2022): 110299.

20. Yanzhou Li?, Feng Qin?, Yanzhou He?, Bo Liu, Conghui Liu, Xuejiao Pu, Fanghao Wan*, Xi Qiao*, Wanqiang Qian*. 2022. The effect of season on Spartina alterniflora identification and monitoring. Frontiers in Environmental Science, 2022, 1044839.

21. Zhang GZ?., Wang CJZ?., Ren XH., Li ZY., Liu CH., Qiao X., Shen SC., Zhang FD., Wan FH., Liu B.*, Qian WQ.*. 2023. Inhibition of invasive plant Mikania micrantha rapid growth by host-specific rust (Puccinia spegazzinii). Plant Physiology, 2023: kiad186.

22. Wang FK.?, Huang YQ.?, Huang ZC., Shen H., Huang C., Qiao X.*, Qian WQ.*. MRUNet: A two-stage segmentation model for small insect targets in complex environments. Journal of Integrative Agriculture, 2023, 22(4): 1117-1130.

23. Zhao X.Y., Li Y.Z., Chen Y.L., Qiao X.*, Qian W.Q.*. Water Chlorophyll a Estimation Using UAV-based Multispectral Data and Machine Learning. Drones, 2023, 7, 2. (SCI, IF: 5.349)

24. Ren XH.?, Zhang GZ.?, Jin MJ., Wan FH., Michael D. D., Qian WQ.*, Liu B.*. 2023. Metabolomics and Transcriptomics Reveal the Response Mechanisms of Mikania micrantha to Puccinia spegazzinii Infection. Microorganisms, 11, 678.

25. Ning Xiaoyu ?, Huang Cong ?, Dong Changhong, Jin Jisu, Qiao Xi, Guo Jianying, Qian Wanqiang, Cao Fengqin*, Wan Fanghao*. RNAi verifications on olfactory defects of an essential biocontrol agent Agasicles hygrophila (Coleoptera: Chrysomelidae) regarding mating and host allocation. Frontiers in Ecology and Evolution, 10, 3389.

26. Chen Y. ?, Huang Y.Q. ?,Zhang Z.Z., Wang Z., Liu B., Liu C.H., Huang C., Dong S.Y., Pu X.J., Wan F.H.*, Qiao X.*, Qian W.Q.*. Plant image recognition with deep learning: A review. Computers and Electronics in Agriculture, 2023, 212(2023): 108072.

代表專利：

1. 錢萬強(qiáng),，劉博，張廣忠,，喬曦,，申時才，萬方浩. 一種抑制薇甘菊快速生長的生物方法（發(fā)明專利）,，授權(quán)日期：2023.01.10,，專利號：ZL 202210230108.4.

2. 喬曦，錢萬強(qiáng),，萬方浩,，彭長連. 入侵植物薇甘菊的自動識別方法及系統(tǒng)（發(fā)明專利），授權(quán)日期：2022.3.25,，專利號：ZL201810572277.X.

3. 錢萬強(qiáng),?劉博,?萬方浩,?申時才,?任行海. 一種薇甘菊柄銹菌擴(kuò)繁方法（發(fā)明專利）,，授權(quán)日期：2021.10.28，專利號：ZL 202010698803.4.

4. 喬曦,，錢萬強(qiáng),，萬方浩，彭長連. 入侵植物的監(jiān)測系統(tǒng)及方法（發(fā)明專利），授權(quán)日期：2021.9.30,，專利號：ZL 201810586568.4.

 

萬方浩,、錢萬強(qiáng)課題組更新于2024年5月