姓名	張 江
職稱	研究員，博士生導(dǎo)師
電話/傳真
電子郵件	[email protected]
實驗室主頁（可選）
研究方向	質(zhì)體介導(dǎo)RNAi抗蟲

 簡歷介紹：

張江,，研究員,，博士生導(dǎo)師,。

2008年獲得中科院上海植物生理生態(tài)研究所博士學(xué)位,，2008-2015年在德國馬普分子植物生理所從事博士后研究,。課題組長期從事植物質(zhì)體工程與抗蟲生物技術(shù)研究,，近年來針對我國重大農(nóng)林害蟲,，建立起了一套基于質(zhì)體介導(dǎo)RNAi抗蟲的技術(shù)體系，為我國RNA生物農(nóng)藥的研制與開發(fā)奠定了基礎(chǔ),。先后主持國家自然科學(xué)基金,，國家重點研發(fā)計劃課題、德國馬普伙伴小組等項目,。以第一或通訊作者身份在Science, Molecular Plant, PNAS, Microbiome, New Phytologist, Trends in Biotechnology, Plant Biotechnology Journal, Journal of Integrative Plant Biology, Plant Cell & Environment,，Plant Journal,，Pest Management Science等雜志上發(fā)表高水平論文40余篇，授權(quán)發(fā)明專利10項,。

 研究領(lǐng)域：

研究方向：

質(zhì)體介導(dǎo)RNAi抗蟲

研究內(nèi)容：

1. 影響質(zhì)體介導(dǎo)RNAi抗蟲效率的主要因子及其調(diào)控機(jī)制,；

2. 害蟲腸道免疫影響RNAi抗蟲效率的分子機(jī)制；

3. 害蟲回避抗蟲dsRNA分子的行為調(diào)控機(jī)制,；

4. 害蟲RNAi抗性發(fā)生的分子機(jī)制及抗性治理,；

研究進(jìn)展：

近年來課題組在質(zhì)體介導(dǎo)RNAi抗蟲的理論與技術(shù)創(chuàng)新領(lǐng)域中取得了一系列突破性原創(chuàng)成果：

1. 創(chuàng)建了基于質(zhì)體介導(dǎo)RNAi的抗蟲新途徑，明確了抗蟲RNAi分子特征,；

2. 闡明了腸道微生物協(xié)同RNAi的抗蟲機(jī)制,；

3. 系統(tǒng)探明了質(zhì)體介導(dǎo)RNAi技術(shù)在防控不同種類害蟲中的有效性及其效率。

 代表論著：

代表論文：

1. Yangcun Li#, Hong Xu#, Wanwan He, Haoling Rong, Shengchun Li, Dae Sung Kim, Peng Han, Yang Yang*, Jiang Zhang*. Silencing of insect dsRNase genes enhances plastid-mediated RNAi efficiency in Colorado potato beetle. Entomologia Generalis. DOI: 10.1127/entomologia/2023/1739

2. Wenbo Xu#, Miao Zhang#, Yangcun Li#, Wanwan He, Shengchun Li, Jiang Zhang* (2022) Complete protection from Henosepilachna vigintioctopunctata by expressing long double-stranded RNAs in potato plastid. Journal of Integrative Plant Biology. doi: 10.1111/jipb.13411.

3. Mengting Wu#, Qi Zhang#, Yi Dong, Zican Wang, Wenqin Zhan, Zebin Ke, Shengchun Li, Lin He, Stephanie Ruf, Ralph Bock, Jiang Zhang* (2022) Transplastomic tomatoes expressing double-stranded RNA against a conserved gene are efficiently protected from multiple spider mites. New Phytologist. 237(4):1363-1373.

4. Dae Sung Kim, Jiang Zhang*. (2022) Strategies to improve the efficiency of RNAi-mediated crop protection for pest control. Entomologia Generalis. DOI: 10.1127/entomologia/2022/1638

5. Kang Ren, Wenbo Xu, Bailing Ren, Jinqiu Fu, Chunmei Jiang*, Jiang Zhang*. A simple technology for plastid transformation with fragmented DNA. Journal of Experimental Botany. 73(18):6078-6088.

6. Yi Dong, Mengting Wu, Qi Zhang, Jinqiu Fu, F. Vanessa Loiacono, Yong Yang, Zican Wang, Shengchun Li, Ling Chang, Ralph Bock, Jiang Zhang*. (2022) Control of a sap-sucking pest insect by plastid-mediated RNA interference. Molecular Plant. 15(7):1176-1191.

7. Wanwan He, Wenbo Xu, Kaiyun Fu, Wenchao Guo, Dae Sung Kim*, Jiang Zhang*. Positional effects of double-stranded RNAs affect RNA interference efficiency in Colorado potato beetle. Pesticide Biochemistry and Physiology. 184:105121.

8. Mengting Wu, Yi Dong, Qi Zhang, Shengchun Li, Ling Chang, F. Vanessa Loiacono, Stephanie Ruf, Jiang Zhang*, Ralph Bock*. (2022) Efficient control of western flower thrips by plastid-mediated RNA interference. Proceedings of the National Academy of Sciences of the United States of America. 119(15):e2120081119.

9. Jinqiu Fu, Shijing Xu, Huan Lu, Fanchi Li, Shengchun Li, Ling Chang, David G. Heckel, Ralph Bock, Jiang Zhang*. (2022) Resistance to RNA interference by plant-derived double-stranded RNAs but not plant derived short interfering RNAs in Helicoverpa armigera. Plant, Cell & Environment. 45(6):1930-1941.

10. Qiqi Chen, Pan Shen, Ralph Bock, Shengchun Li*, Jiang Zhang*. (2022) Comprehensive analysis of plastid gene expression during fruit development and ripening of kiwifruit. Plant Cell Reports. 41(4):1103-1114.

11. Bailing Ren, Jingnan Cao, Yanqiu He, Sheng Yang*, Jiang Zhang*. (2021) Assessment on effects of transplastomic potato plants expressing Colorado potato beetle β-Actin double-stranded RNAs for three non-target pests. Pesticide Biochemistry & Physiology. 178:104909.

12. Jinqiu Fu#, Wenbo Xu#, Wei Huang, Bipeng Wang, Shengchu Li, Jiang Zhang, Ling Chang*. (2021) Importation of taxadiene synthase into chloroplast improves taxadiene production in tobacco. Planta, 253:107.

13. Letian Xu#, Shijing Xu#, Liuwei Sun, Yiqiu Zhang, Jing Luo, Ralph Bock, Jiang Zhang*. (2021) Synergistic action of the gut microbiota in environmental RNA interference in a leaf beetle. Microbiome. 9(1): 98.

14. Shengchun Li, Ling Chang, Jiang Zhang* (2021) Advancing organelle genome transformation and editing for crop improvement. Plant Communications. 2:100141

15. Yuyong Wu, Ling Chang, Chunmei Jiang, Leitian Xu, Jiang Zhang* (2021) Plastid transformation in Poplar: A model for perennial trees. Methods in Molecular Biology. 2317:257-265.

16. Wanwan He#, Wenbo Xu#, Kaiyun Fu, Wenchao Guo, Jiang Zhang*. (2020) Low mismatch rate between double-stranded RNA and target mRNA does not affect RNA interference efficiency in Colorado potato beetle. Insects. 11(7):449.

17. Yi Dong#, Yong Yang#, Zican Wang, Mengting Wu, Jinqiu Fu, Jianyang Guo, Ling Chang*, Jiang Zhang*. (2020) Inaccessibility to double-stranded RNAs in plastids restricts RNA interference in Bemisia tabaci (whitefly). Pest Management Science. 76(9):3168-3176.

18. Wanwan He, Wenbo Xu, Letian Xu, Kaiyun Fu, Wenchao Guo, Ralph Bock, Jiang Zhang*. (2020)  Length-dependent accumulation of double-stranded RNAs in plastids affects RNA interference efficiency in the Colorado potato beetle. Journal of Experimental Botany. 71(9):2670-2677.

19. Shijing Xu#, Yiqiu Zhang#, Shengchun Li, Ling Chang, Yuyong Wu*, Jiang Zhang*. (2020) Plastid-expressed Bacillus thuringiensis (Bt) cry3Bb confers high mortality to a leaf eating beetle in poplar. Plant Cell Reports. 2020, 39(3):317-323.

20. Meiqi Ma, Wanwan He, Shijing Xu, Letian Xu, Jiang Zhang*. (2020) RNA interference in Colorado potato beetle (Leptinotarsa decemlineata): a potential strategy for pest control. Journal of Integrative Agriculture. 19(2):428-437.

21. Yiqiu Zhang, Letian Xu, Shengchun Li*, Jiang Zhang*. (2019) Bacteria-mediated RNA interference for management of Plagiodera versicolora (Coleoptera: Chrysomelidae). Insects. 10(12),415.

22. Yuyong Wu#, Letian Xu#, Ling Chang, Meiqi Ma, Lili You, Chunmei Jiang, Shengchun Li, Jiang Zhang*. (2019) Bacillus thuringiensis (Bt) cry1C expression from the plastid genome of poplar leads to high mortality of leaf eating caterpillars. Tree Physiology. 39(9):1525-1532.

23. Lili You, Qipin Song, Yuyong Wu, Shengchun Li, Chunmei Jiang, Ling Chang, Xinghong Yang*, Jiang Zhang*. (2019) Accumulation of glycine betaine in transplastomic potato plants expressing choline oxidase confers improved drought tolerance. Planta. 249(6):1963-1975.

24. Xiaohui Zhan#, Fengjuan Zhang#, Ziyang Zhong#, Ruhao Chen, Yong Wang, Ling Chang, Ralph Bock, Bihua Nie*, Jiang Zhang*. (2019) Generation of virus-resistant potato plants by RNA genome targeting. Plant Biotechnology Journal. 17(9):1814-1822.

25. Letian Xu#, Jundan Deng#, Fangyuan Zhou, Chihang Cheng, Longwa Zhang*, Jiang Zhang*, Min Lu*. (2019) Gut microbiota in an invasive bark beetle infected by a pathogenic fungus accelerates beetle mortality. Journal of Pest Science. 92:343-351.

26. Letian Xu#*, Yiqiu Zhang#, Shihan Zhang#, Jundan Deng, Min Lu, Longwa Zhang*, Jiang Zhang*. (2018) Comparative analysis of the immune system of an invasive bark beetle, Dendroctonus valens, infected by an entomopathogenic fungus. Development & Comparative Immunology. 88:65-69.

27. Yuyong Wu, Lili You, Shenchun Li, Meiqi Ma, Mengting Wu, Lixin Ma, Ralph Bock, Ling Chang*, Jiang Zhang*. (2017) In vivo assembly in Escherichia coli of transformation vectors for plastid genome engineering. Frontiers in Plant Science. 8:1454.

28. Jiang Zhang, Sher Afzal Khan, David G. Heckel, Ralph Bock*. (2017) Next generation insect-resistant plants: RNAi-mediated crop protection. Trends in Biotechnology. 35(9):871-882.

29. Jiang Zhang, Sher Afzal Khan, Claudia Hasse, Stephanie Ruf, David G. Heckel, Ralph Bock*. (2015) Full crop protection from an insect pest by expression of long double-stranded RNAs in plastids. Science. 347(6225): 991-994.

30. Jiang Zhang, Stephanie Ruf, Claudia Hasse, Liam Childs, Lars B. Scharff, Ralph Bock*. (2012) Identification of cis-elements conferring high levels of gene expression in non-green plastids. The Plant Journal. 72(1): 115-128.

31. Jiang Zhang, Wei Tan, Xinghong Yang, Hongxia Zhang*. (2008) Plastids-expression of choline monooxygenase gene improves salt and drought tolerance through accumulation of glycine betaine in tobacco. Plant Cell Reports. 27(6): 1113-1124.

代表專利：

1. Plant protection from a pest or pathogen by expression of double-stranded RNAs in the plastid. WO2016/097331

2. 一種研究棉鈴蟲幼蟲RNA干擾效率的方法,，專利號：ZL201610664530.5

3. 一種植物質(zhì)體表達(dá)載體的構(gòu)建方法及應(yīng)用,，專利號：ZL201710436614.8

4. 一種利用煙草質(zhì)體高效生產(chǎn)蝦青素的方法,，專利號：201710406641.0

5. 一種高抗白蛾質(zhì)體轉(zhuǎn)Bt基因楊樹新品種的獲得方法,，專利號：ZL201910077185.9

6. srp54k基因及其特異性dsRNA在柳藍(lán)葉甲防治中的應(yīng)用，專利號：ZL201810191662.X

7. 一種高抗柳藍(lán)葉甲質(zhì)體轉(zhuǎn)Bt基因楊樹新品種的獲得方法,，專利號：ZL201910077220.7

8. 一種防治棉鈴蟲的病毒樣顆粒的制備方法,、抵抗棉鈴的方法及其應(yīng)用，專利號：ZL202010212603.3

9. 一種防治桃蚜的質(zhì)體轉(zhuǎn)基因植物的制備方法及其在防治桃蚜中的應(yīng)用,，專利號：ZL202110639601.7

10. 一種表達(dá)病毒樣顆粒的細(xì)胞核轉(zhuǎn)化的轉(zhuǎn)基因植物的制備方法及其在抵抗棉鈴蟲中的應(yīng)用,，專利號：ZL202110491857.8

11. 一種表達(dá)病毒樣顆粒的質(zhì)體轉(zhuǎn)化的轉(zhuǎn)基因植物的制備方法及其在抵抗棉鈴蟲中的應(yīng)用，專利號：ZL202110261758.0

張江課題組更新于2023年9月